MersV1, Ncbi, Merge, bibRecord, 000349

DNA polymerase lambda directly binds to proliferating cell nuclear antigen through its confined C-terminal region.

Identifieur interne : 000349 ( Ncbi/Merge ); précédent : 000348; suivant : 000350

DNA polymerase lambda directly binds to proliferating cell nuclear antigen through its confined C-terminal region.

Auteurs : Noriko Shimazaki [Japon] ; Takaya Yazaki ; Takashi Kubota ; Asami Sato ; Ayako Nakamura ; Shunsuke Kurei ; Shingo Toji ; Katsuyuki Tamai ; Osamu Koiwai

Source :

Genes to cells : devoted to molecular & cellular mechanisms [ 1356-9597 ] ; 2005.

RBID : pubmed:15966901

Descripteurs français

English descriptors

KwdEn :
- Binding Sites, Cell Nucleus (metabolism), DNA Nucleotidylexotransferase (metabolism), DNA Polymerase beta (genetics), DNA Polymerase beta (metabolism), DNA Replication, Green Fluorescent Proteins (metabolism), Humans, Immunoprecipitation, In Vitro Techniques, Microscopy, Fluorescence, Mutagenesis, Site-Directed, Mutation, Proliferating Cell Nuclear Antigen (genetics), Proliferating Cell Nuclear Antigen (metabolism), Protein Binding, Protein Structure, Tertiary.
MESH :
- chemical , genetics : DNA Polymerase beta, Proliferating Cell Nuclear Antigen.
- chemical , metabolism : DNA Nucleotidylexotransferase, DNA Polymerase beta, Green Fluorescent Proteins, Proliferating Cell Nuclear Antigen.
- metabolism : Cell Nucleus.
- Binding Sites, DNA Replication, Humans, Immunoprecipitation, In Vitro Techniques, Microscopy, Fluorescence, Mutagenesis, Site-Directed, Mutation, Protein Binding, Protein Structure, Tertiary.

Abstract

DNA polymerase lambda (Pol lambda) was recently identified as a new member of the family X of DNA polymerases. Here, we show that Pol lambda directly binds to proliferating cell nuclear antigen (PCNA), an auxiliary protein for DNA replication and repair enzymes, both in vitro and in vivo. A pull-down assay using deletion mutants of Pol lambda showed that the confined C-terminal region of Pol lambda directly binds to PCNA. Furthermore, a synthetic peptide of 20-mers derived from the C-terminal region of Pol lambda competes with full-length Pol lambda for binding to PCNA. The residues between amino acids 518 and 537 of Pol lambda are required for binding to PCNA, and are different from the consensus PCNA interacting motif (PIM). Pol lambda associates with PCNA in vivo by immunoprecipitation analysis and EGFP-tagged Pol lambda co-localizes with PCNA as spots within a nucleus using fluorescent microscopy. Through direct binding, PCNA suppressed the distributive nucleotidyltransferase activity of Pol lambda. Pol micro, which also belongs to the family X of DNA polymerases, binds to PCNA by a pivotal amino acid residue.

DOI: 10.1111/j.1365-2443.2005.00868.x
PubMed: 15966901

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Le document en format XML

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<author><name sortKey="Nakamura, Ayako" sort="Nakamura, Ayako" uniqKey="Nakamura A" first="Ayako" last="Nakamura">Ayako Nakamura</name>
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<author><name sortKey="Koiwai, Osamu" sort="Koiwai, Osamu" uniqKey="Koiwai O" first="Osamu" last="Koiwai">Osamu Koiwai</name>
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<term>Cell Nucleus (metabolism)</term>
<term>DNA Nucleotidylexotransferase (metabolism)</term>
<term>DNA Polymerase beta (genetics)</term>
<term>DNA Polymerase beta (metabolism)</term>
<term>DNA Replication</term>
<term>Green Fluorescent Proteins (metabolism)</term>
<term>Humans</term>
<term>Immunoprecipitation</term>
<term>In Vitro Techniques</term>
<term>Microscopy, Fluorescence</term>
<term>Mutagenesis, Site-Directed</term>
<term>Mutation</term>
<term>Proliferating Cell Nuclear Antigen (genetics)</term>
<term>Proliferating Cell Nuclear Antigen (metabolism)</term>
<term>Protein Binding</term>
<term>Protein Structure, Tertiary</term>
</keywords>
<keywords scheme="KwdFr" xml:lang="fr"><term>Antigène nucléaire de prolifération cellulaire (génétique)</term>
<term>Antigène nucléaire de prolifération cellulaire (métabolisme)</term>
<term>DNA nucleotidylexotransferase (métabolisme)</term>
<term>DNA polymerase beta (génétique)</term>
<term>DNA polymerase beta (métabolisme)</term>
<term>Humains</term>
<term>Immunoprécipitation</term>
<term>Liaison aux protéines</term>
<term>Microscopie de fluorescence</term>
<term>Mutagenèse dirigée</term>
<term>Mutation</term>
<term>Noyau de la cellule (métabolisme)</term>
<term>Protéines à fluorescence verte (métabolisme)</term>
<term>Réplication de l'ADN</term>
<term>Sites de fixation</term>
<term>Structure tertiaire des protéines</term>
<term>Techniques in vitro</term>
</keywords>
<keywords scheme="MESH" type="chemical" qualifier="genetics" xml:lang="en"><term>DNA Polymerase beta</term>
<term>Proliferating Cell Nuclear Antigen</term>
</keywords>
<keywords scheme="MESH" type="chemical" qualifier="metabolism" xml:lang="en"><term>DNA Nucleotidylexotransferase</term>
<term>DNA Polymerase beta</term>
<term>Green Fluorescent Proteins</term>
<term>Proliferating Cell Nuclear Antigen</term>
</keywords>
<keywords scheme="MESH" qualifier="génétique" xml:lang="fr"><term>Antigène nucléaire de prolifération cellulaire</term>
<term>DNA polymerase beta</term>
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<keywords scheme="MESH" qualifier="metabolism" xml:lang="en"><term>Cell Nucleus</term>
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<term>DNA nucleotidylexotransferase</term>
<term>DNA polymerase beta</term>
<term>Noyau de la cellule</term>
<term>Protéines à fluorescence verte</term>
</keywords>
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<term>DNA Replication</term>
<term>Humans</term>
<term>Immunoprecipitation</term>
<term>In Vitro Techniques</term>
<term>Microscopy, Fluorescence</term>
<term>Mutagenesis, Site-Directed</term>
<term>Mutation</term>
<term>Protein Binding</term>
<term>Protein Structure, Tertiary</term>
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<term>Immunoprécipitation</term>
<term>Liaison aux protéines</term>
<term>Microscopie de fluorescence</term>
<term>Mutagenèse dirigée</term>
<term>Mutation</term>
<term>Réplication de l'ADN</term>
<term>Sites de fixation</term>
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<front><div type="abstract" xml:lang="en">DNA polymerase lambda (Pol lambda) was recently identified as a new member of the family X of DNA polymerases. Here, we show that Pol lambda directly binds to proliferating cell nuclear antigen (PCNA), an auxiliary protein for DNA replication and repair enzymes, both in vitro and in vivo. A pull-down assay using deletion mutants of Pol lambda showed that the confined C-terminal region of Pol lambda directly binds to PCNA. Furthermore, a synthetic peptide of 20-mers derived from the C-terminal region of Pol lambda competes with full-length Pol lambda for binding to PCNA. The residues between amino acids 518 and 537 of Pol lambda are required for binding to PCNA, and are different from the consensus PCNA interacting motif (PIM). Pol lambda associates with PCNA in vivo by immunoprecipitation analysis and EGFP-tagged Pol lambda co-localizes with PCNA as spots within a nucleus using fluorescent microscopy. Through direct binding, PCNA suppressed the distributive nucleotidyltransferase activity of Pol lambda. Pol micro, which also belongs to the family X of DNA polymerases, binds to PCNA by a pivotal amino acid residue.</div>
</front>
</TEI>
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<DateCompleted><Year>2005</Year>
<Month>09</Month>
<Day>20</Day>
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<DateRevised><Year>2014</Year>
<Month>11</Month>
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<ArticleTitle>DNA polymerase lambda directly binds to proliferating cell nuclear antigen through its confined C-terminal region.</ArticleTitle>
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<Abstract><AbstractText>DNA polymerase lambda (Pol lambda) was recently identified as a new member of the family X of DNA polymerases. Here, we show that Pol lambda directly binds to proliferating cell nuclear antigen (PCNA), an auxiliary protein for DNA replication and repair enzymes, both in vitro and in vivo. A pull-down assay using deletion mutants of Pol lambda showed that the confined C-terminal region of Pol lambda directly binds to PCNA. Furthermore, a synthetic peptide of 20-mers derived from the C-terminal region of Pol lambda competes with full-length Pol lambda for binding to PCNA. The residues between amino acids 518 and 537 of Pol lambda are required for binding to PCNA, and are different from the consensus PCNA interacting motif (PIM). Pol lambda associates with PCNA in vivo by immunoprecipitation analysis and EGFP-tagged Pol lambda co-localizes with PCNA as spots within a nucleus using fluorescent microscopy. Through direct binding, PCNA suppressed the distributive nucleotidyltransferase activity of Pol lambda. Pol micro, which also belongs to the family X of DNA polymerases, binds to PCNA by a pivotal amino acid residue.</AbstractText>
</Abstract>
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<ForeName>Noriko</ForeName>
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<Author ValidYN="Y"><LastName>Yazaki</LastName>
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<ChemicalList><Chemical><RegistryNumber>0</RegistryNumber>
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</Chemical>
<Chemical><RegistryNumber>0</RegistryNumber>
<NameOfSubstance UI="C485184">enhanced green fluorescent protein</NameOfSubstance>
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<Chemical><RegistryNumber>EC 2.7.7.-</RegistryNumber>
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<QualifierName UI="Q000378" MajorTopicYN="Y">metabolism</QualifierName>
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<MeshHeading><DescriptorName UI="D011485" MajorTopicYN="N">Protein Binding</DescriptorName>
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<MeshHeading><DescriptorName UI="D017434" MajorTopicYN="N">Protein Structure, Tertiary</DescriptorName>
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<tree><noCountry><name sortKey="Koiwai, Osamu" sort="Koiwai, Osamu" uniqKey="Koiwai O" first="Osamu" last="Koiwai">Osamu Koiwai</name>
<name sortKey="Kubota, Takashi" sort="Kubota, Takashi" uniqKey="Kubota T" first="Takashi" last="Kubota">Takashi Kubota</name>
<name sortKey="Kurei, Shunsuke" sort="Kurei, Shunsuke" uniqKey="Kurei S" first="Shunsuke" last="Kurei">Shunsuke Kurei</name>
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<country name="Japon"><noRegion><name sortKey="Shimazaki, Noriko" sort="Shimazaki, Noriko" uniqKey="Shimazaki N" first="Noriko" last="Shimazaki">Noriko Shimazaki</name>
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DNA polymerase lambda directly binds to proliferating cell nuclear antigen through its confined C-terminal region.

DNA polymerase lambda directly binds to proliferating cell nuclear antigen through its confined C-terminal region.

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Abstract

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